Differentiation of Entamoeba histolytica and Entamoeba dispar by PCR: a preliminary study in Izmir, Turkey.
نویسندگان
چکیده
The causative agent of amoebiasis is currently attributed to two distinct species (E. histolytica and E. dispar). The aim of this study was to differentiate these species by PCR in stool samples. Isolated genomic DNA was amplified by PCR and band products of 101 bp (E. dispar) were obtained. All seven stool samples were found to be E. dispar, not E. histolytica. Our results demonstrated the significance of E. histolytica/dispar differentiation in the diagnosis of amoebiasis. This study is preliminary to our current research project entitled "Investigation of the prevalence of amoebiasis and Entamoeba species in Izmir and its hinterland".
منابع مشابه
PRELIMINARY COMPARATIVE STUDY OF ENTAMOEBA HISTOLYTICA AND ENTAMOEBA DISPAR BY PCR TECHNIQUE IN IRAN
It has been suggested that two distinct species exist within what was originally known as Entamoeba histolytica and E. dispar. These are Entamoeba dispar for the nonpathogenic and E. histolytica for the pathogenic fonn. Differentiation of these two organisms is of great clinical importance since they are morphologically indistinguishable and both fonns can infect the human intestinal cavity...
متن کاملتمایز انتاموبا هیستولیتیکا و انتاموبا دیسپار با یک واکنش زنجیره‌ای پلی‌مراز
Abstract: Background and Aim: In most part of the world detection of cysts and trophozoites of Entamoeba is based on morphological structure of this species in stool sample by microscopy. However, microscopic examination is unable to distinguish between similar morphological protozoa such as Entamoeba histolytica and Entamoeba dispar. A simple and cost-effective method is needed in medical lab...
متن کاملInvestigation of the prevalence of amoebiasis in Izmir province and determination of Entamoeba spp. using PCR and enzyme immunoassay.
Amoebiasis is a common and life-threatening disease. The discrimination of the pathogenic Entamoeba histolytica from the non-pathogenic Entamoeba dispar could be done by advanced methods such as enzyme immunoassay (EIA) and PCR. The aim of this study was to investigate the prevalence of amoebiasis in Izmir province, and differentiate the Entamoeba species by PCR and EIA. Stool samples of 2,047 ...
متن کاملDifferentiation of Entamoeba histolytica/Entamoeba dispar by the polymerase chain reaction in stool samples of patients with gastrointestinal symptoms in the Sanliurfa Province.
OBJECTIVE We aimed to diagnose amebiasis and also identify Entamoeba histolytica (E. histolytica) and Entamoeba dispar (E. dispar) in patients with gastrointestinal symptoms in an endemic region in Turkey. METHODS Stool samples obtained from 181 patients with gastrointestinal symptoms from the Harran University Hospital of Sanliurfa were examined for the diagnosis of amebiasis by the three me...
متن کاملDetection and differentiation of Entamoeba histolytica and Entamoeba dispar isolates in clinical samples by PCR and enzyme-linked immunosorbent assay.
Differential diagnosis of Entamoeba histolytica (pathogenic) and Entamoeba dispar (nonpathogenic), which are two morphologically identical species of amebae, is essential both for treatment decision and public health knowledge. The study reported here was designed to choose a reference differentiation technique. Stool samples (n = 95) were tested by microscopy, TechLab enzyme-linked immunosorbe...
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ورودعنوان ژورنال:
- The new microbiologica
دوره 30 1 شماره
صفحات -
تاریخ انتشار 2007